Four search programs in PhosphoPOINT

Four search programs in PhosphoPOINT

(A) A database search by kinase gene symbol, Entrez GeneID and SwissProt terms

(B) Using user-defined or about 300 known kinase/phosphatase substrate/binding motifs to search for all phospho-peptides

The result of Database Search - 1

Annotation and visualization in PhosphoPOINT -1

At the below left, to visualize the human kinase (e.g. AURKA) interacting proteins, we have used various colors to represent the different types of nodes (circle)/edges (line). The different node colors represent the phosphorylation status of each protein (e.g. red stands for this protein containing ser/thr phosphorylation sites). Edges represent the relationship between kinase and its interacting (phospho)-proteins/substrates. (Right) For each kinase and its interacting (phospho)-proteins/substrates pairs, we have calculated the Pearson correlation (solid rectangle) of each pair using the NCI60 and GNF microarray datasets. The red solid rectangle means a highly positive correlation (r>=0.7) between each pairs. The similarity of cellular component terms from GO for each kinase pairs is also illustrated in the third line with a hollow rectangle.

(A) Gene detail information

(B) Gene Expression data from NCI60 and GNF datasets

The result of Database Search - 2

Annotation and visualization in PhosphoPOINT -2

(A) Phosphorylation sites information

(B) the three-dimensional structure information flanking with the phosphorylation sites